1987 Volume 78 Issue 10 Pages 1746-1753
For the purpose of early diagnosis of bladder tumor and its maligant potential, immunocytochemical demonstration of TPA and ABH antigen by using urothelial exfoliated cells was performed. Indirect immunoperoxidase technique was utilized for detecting urinary TPA and ABH staining, which was compared with tissue TPA, ABH and RIA urinary TPA.
The resected specimens were composed of 45 bladder tumors and 10 non-cancerous tissues. In individual cells, urinary TPA and ABH were present in the cytoplasma and cell membrane. The positive rate of urinary TPA staining was related to RIA urinary levels, but not to localization of tissue TPA. The positive rate of urinary TPA staining for bladder tumors was 75.6% (34/45), which was superior to that (62.2%) of conventional urinary cytology. There was a good correlation between the positive rates of urinary TPA staining and tumor growth (grade, stage).
On the other hand, the incidence of recurrence following radical TUR-bt was significantly correlated to tissue ABH antigens, but not to pathological grade. Though idependent interpretation was performed, loss of ABH antigens by exfoliated cells was accordant with that on tissue sections.
Detection of TPA and ABH antigens by urothelial exfoliated cells is a non-invasive, reliable test that could be used for screening and surveillance of outpatients with bladder tumors. It seems, therefore, that this method enables us to monitor patients with bladder tumors by porspective screening.
