AN EXPERIMENTAL STUDY OF ATTACHMENT TO COLLAGEN, UTILIZING ESTABLISHED CELL LINE OF HUMAN URINARY BLADDER CANCER (T24)
1987 Volume 78 Issue 10 Pages 1754-1763
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1987 Volume 78 Issue 10 Pages 1754-1763
An attachment assay to collagen substrates, using cell strain T24 which is an established bladder cancer, transitional cell carcinoma, was performed. A method which examines the cell spreading by determining the time maximum cellular long diameter as a parameter reflecting cellular attachment was also devised. An experiment on the involvement of fibronectin, regarded as an attachment factor, and laminin in the attachment of cancer cell to collagen substrates was made. The results obtained were as follows.
1) In the presence of MEM added albumin and in the presence of MEM with added serum and albumin, the attachment was significantly higher to type I collagen and type IV collagen than to plastics. Change in the rate of attachment due to the addition of serum showed an increasing tendency for type I collagen.
2) High rates of cell spreading were observed for type I collagen and type IV collagen.
3) With the addition of cycloheximide, the attachment to type I collagen and type IV collagen decreased.
4) With and urthout the addition of fibronectin, the attachment to type I collagen showed an increasing tendency.
5) With the addition of laminin, the attachment to type IV collagen showed an increasing tendency.
The rate of cell spreading of T24 exhibited a similar kinetic behavior to the rate of cellular attachment, suggesting the usefulness of the rate of cell spreading as a measure of cellular attachment.
