1988 Volume 79 Issue 1 Pages 143-149
The chemosensitivity of human bladder tumor cell line T-24 to 4 anticancer drugs (CDDP, MTX, VLB, ADM) has been studied by subrenal capsule assay (SRC), human tumor-nude mice xenograft system and human tumor clonogenic assay (HTCA), and the usefulness of these 3assays is discussed. The sensitive drugs are VLB in SRC, VLB and ADM in the nude mice system. No drug is sensitive by HTCA.
In HTCA, ADM can be judged effective with higher standards of drug concentration and VLB is also effective on continuous drug exposure. MTX is resistant in in-vavo assay and with any standard of drug concentration or continuous drug esposure in HTCA. These results suggest that 1) in in-vavo assay, drug dose to mice should be modified to correlate to clinical drug concentration in humans, 2) in HTCA, drug concentration and time of drug exposure should also be modified with each drug, taking into consdderation its pharmacokinetics and functional character. SRC assay is well correlated to xenograft in nude mice system, and will be one of the useful assays for clinical use, superior to the present in-vavo assays.
