2025 Volume 10 Issue 2 Pages 35-43
Protein phosphorylation is a representative post-translational modification that regulates intracellular signal transduction, and its abnormalities play a central role in many cancers. Phosphoproteome analysis is a powerful method for revealing dynamic changes in signaling networks that cannot be fully captured by gene mutation or expression analysis. This review article describes the development of a phosphoproteome analysis method that achieves both analytical depth and rapidity. We demonstrate its effectiveness for developing novel cancer therapies through examples validated in cultured cells, surgically resected specimens, and endoscopic biopsy specimens. Finally, I discuss future directions.
