Pathogenicity and diagnostic sensitivity of culture media for identifi cation of Trichomonas gallinae in Domestic Pigeons of Lahore, Pakistan

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Avian trichomoniasis is a serious infection caused by a trophozoite of Trichomonas gallinae (Rivolta, 1878) in domestic pigeons (Columba livia domestica). Different culture media are currently used for the T. gallinae identification in these hosts. Two commercially available cultured media, namely modified Diamond’s medium and modified thioglycolate medium, were used to identify T. gallinae growth. Microscopic examinations of oral swabs of pigeons were carried out post inoculation by incubating at various temperature. Significant difference between incubation time found for modified Diamond’s medium and Modified thioglycolate medium have been tested. Modified thioglycolate medium had been tested as a readily available, low-cost substitute for the Diamond’s medium. Diamond’s medium is considered as “gold standard” for identification T. gallinae in animals. However, modified thioglycolate medium was found to be practically efficient than Diamond’s medium in recovering the parasites from specimens and may provide a readily available, low-cost substitute for the Diamond’s medium. The current research also demonstrated the histopathology associated with infection of T. gallinae.