Diagnosis Of Trypanosoma evansi Infection Among Sudanese Camels Imported To Egypt Using Card Agglutination Test (CATT) And Antigen Detection Latex Agglutination Test (Suratex)

抄録

A total of 125 imported Sudanese camels were tested for Trypanosoma evansi infection to determine the prevalence of camels with chronic disease that may act as an exotic source of infection. The research employed direct blood smear examination, card agglutination test (CATT/T. evansi) for antibody detection and latex agglutination test (Suratex) for the detection of circulating trypanosomal antigens. Blood smear examination detected patent parasitemia in five camels (4.0%) (two cases were CATT negative). Thirty six camels (28.8%) were CATT positive, while forty five(36.0％) were positive for circulating antigens. Thirty one cases (24.8%) were positive for both tests. Five (4.0%) CATT reactors were negative for trypanosomal antigens. However, 14(11.2％) camels with circulating trypanosomal antigens, including one case with patent parasitemia, tested CATT negative. Samples of these camels, as well as camels with parasitemia were tested for antibodies to common trypanosomal antigens using indirect hemagglutination test (IHA) using T. brucei gambiense antigen. Eleven (78.60%) of the 14 CATT-negative camels had IHA titers ranging from 1/64-1/2048 (including the case with parasitemia) giving concurrence with Suratex results. All five cases with patent parasitemia were positive for IHA. Detection of circulating T. evansi antigens using Suratex was found to be a more sensitive and reliable means of practical diagnosis of camels with chronic latent infection.