Abstract
The purpose of the present study is to clarify the relationship between the constitution and the blood types of chicken classified with natural antibodies by Suzuki.
The present paper reports on the blood types of chicken investigated by the elution test and the immunochemical characteristics analyzed by method of 2-mercaptoethanol treatment, ultracentrifugation, heat-treatment, immunoelectrophoresis and diethylaminoethyl cellulose anion exchanger.
The results obtained were as follows:
1) The blood cells (FsI and FsII) were reagglutinated by the antibodies eluted from antigen antibody complex.
2) The antibodies eluted from antigen-antibody complex were obsereved in β-globulin zone by immunoelectrophoresis.
3) The activity of antibodies was recognized in the most under domain of fractions obtained by centrifugation.
4) The agglutination turned to disappear by the treatment of 2-mercaptoethanol.
5) The protein, appearing at the end of a DEAE-chromatogram and being responsible for the capacity of the sera to agglutinate red cells treated by papain.
6) It is considered that the major constituent in these antibodies was IgM (19S, γM) type globulin.
