Immunogenetic Studies in Japanese Quail
2. The Effects of PVP as an Enhancing Agent in Quail Iso-hemagglutination Tests
1970 Volume 7 Issue 4 Pages 182-185
Details
1970 Volume 7 Issue 4 Pages 182-185
The effectiveness of polyvinylpyrrolidone (PVP, K-90) as enhancing agent in quail isohemagglutination reactions are tested. A comparison of the saline isotonic solution with the PVP technic reveals a comparable degree of sensitivity in the enhancement of hemagglutinin titer.
The results are summarized as follows:
1) It is observed that 0.1 percent PVP and antiserum mixture technic develops successfully the agglutination titers of quail cells by one or two tubes regardless of the genetic systems. However, 0.1 percent PVP saline is effective to antiserum showing a titer or higher.
2) It is observed that 0.3 or 0.5 percent PVP saline is useful to enhance antiserum of 1:16 or less titer.
3) The incomplete agglutinins in the used iso-mmune sera are not detected with the PVP technic.
4) The relative simplicity in the routine laboratory use and a saving in iso-antiserum are the added advantages of the PVP technic.
