骨格筋の恒常性維持に対する機能性食品素材の役割

抄録

The polyamines, a functional foods material are considered to be essential growth factors in virtually all cells. The proposed roles of polyamines are the functioning of ion channels, nucleic acid packaging, signal transduction, cell proliferation, and differentiation, as well as regulation of gene expression. In skeletal muscle, regulation of polyamine levels is associated with muscle hypertrophy and atrophy, yet the underlying mechanisms of polyamine actions are not well defined. Here, we studied how polyamines may affect the proliferation and/or differentiation of murine myoblast progenitor C2C12 cell line. Upon polyamine treatment of C2C12 cells during induction of myogenic differentiation, the number of myotubes significantly increased. Morphologically, polyamine-treated C2C12 cells exhibited elongated cell body and became multi-nucleated myotubes. On the other hand, the polyamine did not have influence on myoblasts proliferation. Furthermore, compensatory muscle hypertrophy of C57BL6 mice underwent sciatic nerve transection of the left hindlimb was enhanced by administration of polyamines. Therefore, our study demonstrates that polyamines may play an important role in skeletal muscle homeostasis by enhancing myogenic differentiation.