尿サンプル由来膀胱癌オルガノイド培養法を用いた抗がん剤感受性検査の開発

抄録

【Background】

Currently, anti-cancer drugs are used in veterinary medicine for a variety of tumors, but sensitivities to the drugs vary among patients, even for the same type of tumor. Although standard protocols exist, their efficacy is uncertain. We have developed a three-dimensional organoid culture model of dog bladder cancer using urine samples. The model also can be used as a spontaneous model of human muscle-invasive bladder cancer However, it remains unclear whether the sensitivity to anti-cancer drugs in organoids correlates with actual treatment effects.

【Methods】

We analyzed the correlation between anti-cancer drug sensitivity of organoids and clinical effects by measuring the sensitivity to anti-cancer drugs (vinblastine, mitoxantrone, and carboplatin) using organoids from BC diseased dogs and following up their treatment in the clinic.

【Result】

The organoids were observed in basal, luminal, and neural forms, depending on the patient. The neural types showed resistance to anti-cancer drugs. Vinblastine treatment most stably reduced cell viability, whereas mitoxantrone or carboplatin treated organoids were found to be resistant in some dogs. Long-term vinblastine treatment of dogs which showed high sensitivity to vinblastine in organoids resulted in significant cancer regression.

【Discussion】

These results suggest that urine sample-derived BC organoids can be used as a useful tool for pre-treatment assessment of anti-cancer drug sensitivity in dogs with BC.