主催: 第94回日本薬理学会年会
会議名: 第94回日本薬理学会年会
回次: 94
開催地: Sapporo
開催日: 2021/03/08 - 2021/03/10
Hypothesis Interstitial cystitis (IC) is a chronic inflammatorydisease characterized by pain in the bladder and voiding symptoms. To date,there are no effective treatments for IC. We investigated whether filteredadipose-derived stem cell lysate (FADSCL) alleviates IC in a rat model ofHCl-induced cystitis.
Materials and methods Rat adipose-derived stem cells were collectedfrom the subcutaneous fat of rats, and FADSCL (lysate of 1×107 cells/PBS) wasprepared. Female Fisher 344 rats, aged eight weeks, were divided into thesham+PBS (sham, n=10), HCl+PBS (HCl, n=12), and HCl+FADSCL (FADSCL, n=9) groups.Saline or 0.1 M HCl (250μl/body) was intravesically administered from atransurethral catheter. Bladder function was investigated after one week bycystometrography. A sample of bladder tissue was used for RNA-seq analysis.
Results The intercontraction intervals (ICIs) in the HCl group weresignificantly shorter than those in the sham group (P<0.01), while the ICIsof the FADSCL group were significantly longer than those of the HCl group(P<0.01). There was no significant difference between the groups with respectto the maximum voiding pressure and bladder weight. RNA-Seq analysis revealed anincrease in immune and inflammatory signaling in the HCl group compared to thatin the sham group, and these signals were suppressed in the FADSCL group.
Conclusion FADSCL might alleviate IC via anti-inflammatory actionsand suppress the immune response. Thus, FADSCL could serve as a noveltherapeutic agent for IC.
