脳オルガノイドのMEA計測

抄録

Micro-electrode array (MEA) assay using in vitro human iPSC-derived neurons is expected as one of the assessments for predicting seizure liability of drugs. However, it is necessary to approach the in vitro to in vivo extrapolation (IVIVE). As one approach to IVIVE, an assessment method using a human brain organoid that mimics the three-dimensional structure is considered to be effective. In this study, we attempted to detect the response to convulsants by MEA measurement using human cerebral organoids. We detected the responses to convulsants in cerebral organoids and found that features appear in low frequency components of MEA data. Wavelet analysis revealed that the frequency intensity from the θ wave to the β wave component significantly increased in a dose-dependent manner. This is a result that enables comparison with in vivo brain waves. This study demonstrated that MEA measurement using human brain organoids may be a method that can approach in vitro to in vivo extrapolation in prediction of seizure liability of drugs.