85As2胃がん細胞播種がん悪液質モデルにおける白血病阻止因子（LIF）による骨格筋委縮作用：C2C12骨格筋細胞を用いた解析

抄録

Cancer cachexia, a syndrome characterized by anorexia and body weight loss due to low fat-free mass levels including reduced musculature, markedly decreases the quality of life in cancer patients. Although stomach cancer patients have the highest incidence of cachexia, few experimental models for the study of stomach cancer cachexia have been established. We previously established a murine model of cancer cachexia by implantation of human stomach cell line 85As2 showing symptoms of cancer cachexia observed in cancer cachexia patients, and we found leukemia inhibitory factor (LIF) as one of the cachexia-inducing factor. In this model, plasma concentrations of LIF are increased in association with the changes of hypothalamic orexigenic and anorexigenic peptide mRNA levels, as well as decreased food intake and loss of body weight with musculature reduction. However, it is unclear whether increased levels of LIF secreted from 85As2 cells have atrophic effects to musculature. In the present study, we sought to determine the atrophic effects of LIF in in vitro experimental system using differentiated C2C12 cells derived from murine skeletal muscle cells. The results with the cells will be presented.