Host: The Japanese Pharmacological Society
Name : The 96th Annual Meeting of the Japanese Pharmacological Society
Number : 96
Location : [in Japanese]
Date : November 30, 2022 - December 03, 2022
Capillary electrophoresis-mass spectrometry (CE-MS) is a powerful tool for the comprehensive profiling of ionic biomolecules, including neurotransmitters such as glutamic acid (Glu), γ-aminobutyric acid (GABA), and other monoamines. However, CE-MS often lacks sensitivity due to the limitation in the sample injection volume (~ few nL), and it is not easy to analyze neurotransmitters of which amount is usually quite limited in the brain. In order to address this issue, we have developed highly efficient online sample preconcentration methods, which provide up to 2,000-fold higher sensitivity without losing separation performance [1,2]. By applying these techniques, we have achieved trace bioanalysis like single cell metabolome analysis [3], that shows the great potential of our CE-MS for exploring the trace brain molecules. In this symposium, I will introduce our recent studies on the ultra-sensitive analysis of brain molecules, especially focusing on neurotransmitters like Glu and GABA.
[1] T. Kawai et al., Chromatography, 2017, 38, 1.
[2] T. Kawai et al., J. Chromatogr. A 2018, 1565, 138.
[3] T. Kawai et al., Anal. Chem. 2019, 91, 10564.
