脳卒中後てんかん加齢マウスモデルとしての光誘発性血栓法の有用性

抄録

Stroke is a prevalent medical condition, a leading cause of disability and the third most common cause of death. Post-stroke seizure/epilepsy (PSS/E) is a common complication, accounting for 30–40% of epilepsy cases in older adults. In this study, we have discussed GABA_A receptor-mediated seizure susceptibility after photothrombotic (PT) cerebral stroke in aged mice. Generally, young adult mice aged around 10 weeks are widely used in stroke experiments. Our study is based on the facts that most strokes are diagnosed in the older adults and PSS/E is a common complication of stroke. Hence, we utilized the PT model of cerebral ischemia in aged mice. For this purpose, we examined susceptibility to seizures to use low-doses of pentylenetetrazol (PTZ) injections, and seizures were monitored by video and EEG. Susceptibility to PTZ in aging mice increased relative to that in younger adults. A month after PT stroke, older mice still exhibited severe late-onset convulsive seizures. Anti-seizure medications like levetiracetam showed temporary seizure control in such cases. Thus, GABA_A receptor-mediated seizures susceptibility post-PT stroke in aging mice may be an effective method for examining the effects of anti-seizure medication.