トリコプレインを介した一次線毛動態制御の組織再生における役割

抄録

Primary cilia are immobile structures that extend from the surface of various cells, and it is known that they are involved in signal transduction from the extracellular environment. Signal transduction through primary cilia is associated with various intracellular processes, and it has been reported to function in tissue regeneration; however, the detailed mechanism remains unclear. Trichoplein (Tchp) is localized in the basal bodies of primary cilia and functions as a suppressor of primary cilia formation through the activation of Aurora A kinase. In this study, we created Tchp knockout zebrafish to investigate the role of Tchp in tissue regeneration. We performed a fin injury regeneration experiment. We found that the Tchp-knockout zebrafish showed a higher regenerative ability than the wild type. Furthermore, we conducted a quantitative proteome analysis using regenerated zebrafish fins to identify novel genes involved in Tchp-mediated tissue regeneration. We identified 8,756 proteins, of which the expression of 186 proteins was found to be significantly altered in the Tchp knockout zebrafish. Additionally, among these variable proteins, we evaluated the functions of some genes potentially involved in tissue regeneration. We will present the results of the evaluation.