ミクログリアが脳梗塞の海馬歯状回顆粒細胞の樹状突起スパインに与える影響

抄録

Microglia contribute to synaptic pruning by synaptic engulfment, which is dependent on neuronal activity. Our middle cerebral artery occlusion (MCAO) mice show an upregulation of neuronal activity in the hippocampal dentate gyrus (DG). Thus, we investigated the changes in dendritic spines in DG granule cells following cerebral ischemia and microglial contribution to it. We showed that the number of dendritic spines of DG granule cells in MCAO mice was lower than that in sham mice. However, microglial depletion with CSF1R inhibitor (PLX3397) inhibited the MCAO-induced reduction of the dendritic spine. These results suggest that microglia are involved in the decrease in the number of dendritic spines of DG granule cells following cerebral ischemia.