主催: The Japanese Pharmacological Society, The Japanese Society of Clinical Pharmacology
会議名: WCP2018 (18th World Congress of Basic and Clinical Pharmacology)
開催地: Kyoto
開催日: 2018/07/01 - 2018/07/06
Stress is caused by adverse social environment, and often causes emotional changes including depression and elevated anxiety. Using social defeat stress in mice, we previously reported that social defeat stress preferentially activates the dopaminergic pathway projecting to medial prefrontal cortex (mPFC), and that repetition of social defeat stress attenuates this activation, leading to social avoidance. However, the mechanisms of dopamine action in the mPFC in regulating the resilience to social defeat stress remain elusive. Here we show a role of dopamine D1 receptor in mPFC excitatory neurons for the stress resilience. Repeated social defeat stress selectively reduced mRNA expression of D1 receptor in mPFC of susceptible mice. Knockdown of D1 receptor in whole neuronal population or excitatory neurons in mPFC by viral delivery of artificial microRNA facilitated induction of social avoidance by social defeat stress. Single social defeat stress induced D1 receptor-mediated ERK phosphorylation and c-Fos expression in mPFC neurons. Single social defeat stress increased arborization and spine density of apical dendrites of mPFC superficial layer pyramidal neurons in a D1 receptor-dependent manner. Furthermore, transcriptome analysis showed that single social defeat stress alters expression of multiple genes through D1 receptor in mPFC, some of which are involved in regulation of neuronal morphology. These results suggest that D1 receptor mediates acute stress-induced dendritic growth of excitatory neurons and gene expression changes associated with stress resilience in mPFC.