主催: The Japanese Pharmacological Society, The Japanese Society of Clinical Pharmacology
会議名: WCP2018 (18th World Congress of Basic and Clinical Pharmacology)
開催地: Kyoto
開催日: 2018/07/01 - 2018/07/06
BACKGROUNDS:
Pyelonephritis is generally the result of an ascending infection of the urinary tract, and most common microorganism is Escherichia coli. In the last 5 years 3709 children were admitted in Nephrological department of The State Mathernal and Children's Scientific Center, but between them 741 (19.97%) children were caused by pyelonephritis.
OBJECTIVES:
To indicate the prevalence type and the etiological bacterial, and also the antibiotic susceptibility and resistance types of causative pathogens.
METHODS:
We used retrospective and prospective study design on patient's history of children (N=283), which has been diagnosed pyelonephritis. From patient's history we used biochemical, blood count and urine analysis. Strains were identified by API tests strip, and antibiotic susceptibility was determined by disk diffusion method. Types of the beta lactamase were determined by PCR using the blaTEM, blaSHV and blaCTX-M specific primers. WHONET5.1 programm was used for the analysis of susceptibility testing according to the WHO recommendation.
RESULTS:
From total of 283 children 172 cases (60.77%) were a culture positive. Among culture positive cases, E.coli 103 (59.88%), Enterobacter 46 (26.74%), Proteus 11 (6.40%), Pseudomonas aerugenosa 8 (4.65%). Antibiotic susceptibility testing covered 121 bacterial species to 18 types of antibiotics. The E.coli was sensitive to imipenem (98.1%), amikacin (90.4%), cefoxitine (67.9%), nitrofurantione (67.8%) and ceftazidime (64.2%). Using PCR test, 18 isolates contained blaTEM types, 11 strains contained blaSHV types and 16 strains contained blaCTX-M type genes. blaTEM type resistance can divided into 5 groups, from them 2 groups had the resistance to single beta-lactamic antibiotics, but 3 groups had to both beta-lactamic and aminoglycoside antibiotics. blaSHV type resistance had 2 groups antibiotics, these were single beta-lactamic and aminoglycoside antibiotics. blaCTX-M type resistance had 2 groups, these were both beta-lactamic and aminoglycoside antibiotics.
CONCLUSION
1. By our study indicated that the E.coli (59%) was the main cause.
2. By PCR were identified the blaTEM, blaSHV and bla CTX-M type of the beta-lactamase antibiotic resistant genes.
