主催: The Japanese Pharmacological Society, The Japanese Society of Clinical Pharmacology
会議名: WCP2018 (18th World Congress of Basic and Clinical Pharmacology)
開催地: Kyoto
開催日: 2018/07/01 - 2018/07/06
Nanotechnology provides a novel strategy for the delivery of anticancer drugs. In this study, titanium dioxide coated gold nanorods (GNR/TiO2) was used as the drug carrier for gambogic acid in order to improve its anticancer effect. Biocompatibility and cellular uptake of GNR/TiO2 was studied in human glioblastoma U-87 MG cells. Cell viability was evaluated by ATP assay and calcein AM staining. LysoSensor Green DND-189 and Hoechst 33342 were used to analyze the intracellular location of GNR/TiO2. The in vitro anticancer effect of gambogic acid loaded nanoparticles was compared with free drug. The results showed that GNR/TiO2 is biocompatible, and they are localized at the intracellular acidic compartments of endosomes and lysosomes. The intracellular drug content delivered via GNR/TiO2 was 6 fold higher than the free form, thus dramatically enhancing the anticancer effect of gambogic acid. Furthermore, mild photothermal therapy also showed synergistic effect with the drug. Our study suggested that GNR/TiO2 is a promising anticancer drug carrier.
Acknowledgements: The project was supported by the Macao Science and Technology Development Fund (FDCT) (Project No.: 014/2014/A1)
