主催: The Japanese Pharmacological Society, The Japanese Society of Clinical Pharmacology
会議名: WCP2018 (18th World Congress of Basic and Clinical Pharmacology)
開催地: Kyoto
開催日: 2018/07/01 - 2018/07/06
Background:
A cough is one of the most common adverse reactions of angiotensin-converting enzyme (ACE) inhibitors leading to drug discontinuation. In Thailand, cough developed in 66% of patients during enalapril treatment. ACE inhibitor-induced cough relating to a decrease in ACE activity and genetic variation of ABO blood group systems has been reported in many population. However, the research in Thai population has not been studied. Therefore, the objective of this study was to investigate the relationship between ABO polymorphisms and ACE activity in Thai subjects.
Methods:
Blood was collected from 100 healthy Thai volunteers for DNA isolation and ABO grouping. The serum ACE activity was assessed by a spectrophotometric method and FAPGG was used as a substrate. The real-time PCR based allelic discrimination assays was used to genotype for ABO rs495828G>T and rs505922C>T. Descriptive data were expressed as median and interquartile range (IQR). Categorical data were compared using Kruskal-Wallis test.
Results:
ABO rs495828G>T and rs505922C>T SNPs were significantly associated with ACE activity (p = 0.016 and 0.043) respectively. Subjects with rs495828 GT genotype had lower ACE activity (median: 28, IQR: 22-32 U/L) than GG genotype (median: 36, IQR: 26-45 U/L). In addition, Lower ACE activity (median: 26, IQR: 18-37 U/L) were observed in rs505922 TT genotype compared to CT genotype (median: 32, IQR: 27-42 U/L).
Conclusion:
ABO rs495828 and rs505922 SNPs showed relationships with ACE activity in Thai subjects. Further studies are required to confirm the influences of ABO polymorphisms on ACE activity and cough in Thai patients receiving ACE inhibitors.
