抄録
Background. Systemic lupus erythematosus is an autoimmune disease with several clinical manifestations such as arthritis, thrombocytopenia, hemolytic anemia, thrombosis, and nephropathy. Stachytarpheta jamaicensis (L) Vahl. is one of Indonesian traditional herb that can use as an immunomodulator. This research objective was to evaluate the effect of oral administration of water extract S. jamaicensis herbs on a hematological profile, inflammatory response, kidney function, and organ indexes of animal models of systemic lupus erythematosus.
Methods. Extract of S. jamaicensis was made using boiled water. Animal models of systemic lupus erythematosus was inducted by 0.5 mL pristane i.p. After 4 weeks of pristane induction, the extract was administered daily per oral at doses of 11.25, 22.5, and 45 mg/kg bw for 6 weeks. Methylprednisolone 1.4 mg/kg bw i.p was used as a reference. The inflammatory response was evaluated using carrageenan-induced paw edema in rats. The hematological profile was detected using auto hematology analyzer, and hematology parameter was leucocyte count and its differentiate (lymphocyte, mid-sized cell, granulocyte), erythrocyte, hemoglobin, hematocrit, and thrombocyte. Measurement of kidney function was creatinine serum and proteinuria.
Results. The results showed that water extract of S. jamaicensis at doses of 11.5 and 22.5 mg/kg bw had antiinflammatory activity in acute carrageenan-induced paw edema compared to control group (p<0.05) and the extract at dose 11.5 mg/kg bw gave the higher inhibiting action than other doses. The extract at all dose affected the total amount of erythrocyte, leucocyte, lymphocyte, and thrombocyte compared to control group (p<0.05). The extract at all dose increased leucocyte count, leucocyte differentiates and thrombocyte but decreased erythrocyte, hemoglobin, and hematocrit. The extract at all dose reduced serum creatinine (p<0.05), and only at a dose of 11.25 mg/kg bw reduced proteinuria compared to control group (p<0.05). The extract at all dose increased the kidney and spleen index compared to control group.
Conclusion. Oral administration of water extract of S. jamaicensis at a dose of 11.25 mg/kg bw for 6 weeks could inhibit the inflammatory response, increase the risk of anemia and reduce the possibility of thrombocytopenia and kidney damage on animal models of systemic lupus erythematosus.
