Abstract
Early study investigated that curcumin disturbed folliculogenesis by decreasing estrogen and expression of reseptor estrogen (ER) beta in FSH- or LH-stimulated granulosa cells of pig medium follicles. Presence of FSH, LH, PGF2 alpha, estrogen and ER beta are crucial in the growth and development of granulosa cells. Traditionally Curcuma longa L, as the major resources of curcumin, is consumed during folliculogenesis to prevent pregnancy. By understanding that C. longa L also affects at the same targets it would be most useful additional information for anyone who will develop it to drug or practice traditional medicine. The aim of the study was to determine effect of ethanolic extract of C. longa L (ECL) on estrogen secretion and ER beta expression in FSH-, LH-, and PGF2 alpha-stimulated pig granulosa cells of medium follicles.
The study was an experimental laboratory study with posttest-only control group design. Granulosa cells were isolated from medium follicles of pig ovaries and sub cultured in medium culture. Study was done on 16 different treatment groups which divided into 4 different stimulation groups (unstimulated, FSH-stimulated, LH-stimulated, and PGF2 alpha-stimulated granulosa cells). Each stimulated group then divided into 4 treatment groups which were untreated group and ECL treated groups in 3 graded concentrations. The estrogen level and ER beta expression were analyzed by enzymeimmunoassay.
Compare to the control group, estrogen level was significantly higher in granulosa cells treated with highest concentration of ECL but ECL no influenced the ER beta expression. Estrogen level is not and ER beta expression is significantly deceased in FSH- or LH-stimulated granulosa cells treated by ECL compare to those untreated. Estrogen level and ER beta expression in PGF2 alpha-stimulated granulosa cells treated by highest concentration of ECL was significantly higher than in those untreated.
ECL was able to disturb folliculogenesis by decreasing expression of ER beta in FSH- or LH-stimulated granulosa cells of pig medium follicles without disturbing steroidogenesis. Therefore, ECL may a potential agent for antifertility.
