Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11

Yuji KAWAMATA; Junko ISHIJIMA; Satoshi TANAKA; Masato SASAKI; Jumpei ENAMI; Hideaki TOJO; Chikashi TACHI

doi:10.1262/jrd.41.331

Original Articles

Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11

Yuji KAWAMATA, Junko ISHIJIMA, Satoshi TANAKA, Masato SASAKI, Jumpei ENAMI, Hideaki TOJO, Chikashi TACHI

Author information

Yuji KAWAMATA
Laboratory of Applied Genetics, Department of Bio-Regulatory Systems, Institute of Animal Resource Science, School of Agriculture and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
Junko ISHIJIMA
Laboratory of Applied Genetics, Department of Bio-Regulatory Systems, Institute of Animal Resource Science, School of Agriculture and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
Satoshi TANAKA
Laboratory of Applied Genetics, Department of Bio-Regulatory Systems, Institute of Animal Resource Science, School of Agriculture and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
Masato SASAKI
Zenyaku Kogyo Co., Ltd., 2-33-7, Oizumi-machi, Nerima-ku, Tokyo 177, Japan
Jumpei ENAMI
Zenyaku Kogyo Co., Ltd., 2-33-7, Oizumi-machi, Nerima-ku, Tokyo 177, Japan
Hideaki TOJO
Laboratory of Applied Genetics, Department of Bio-Regulatory Systems, Institute of Animal Resource Science, School of Agriculture and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
Chikashi TACHI
Laboratory of Applied Genetics, Department of Bio-Regulatory Systems, Institute of Animal Resource Science, School of Agriculture and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan

Keywords: Long form of prolactin receptor, HC11, Whey acidic protein, Mammary gland, Prolactin

JOURNAL FREE ACCESS

1995 Volume 41 Issue 4 Pages 331-338

DOI https://doi.org/10.1262/jrd.41.331

Details

Abstract

The prolactin (PRL)-responsive cell line, HC11, derived from normal mammary epithelial cell line COMMA-1D, expressed the long form of prolactin receptor (L-PRLR) mRNA, though the level was considerably lower than that in the mammary gland of the normal lactating mouse. HC11 cells expressed whey acidic protein (WAP) mRNA, when they were cultured to confluence and 'in vitro lactogenesis' was induced by the addition of PRL, insulin and dexamethasone. The levels of PRL-induced WAP expression, as measured by reverse transcriptase mediated polymerase chain reaction (RT-PCR), increased in a dose-dependent manner, as PRL concentrations were raised from 0.05 μg/ml to 50 μg/ml. Treatment of HC11 cells with human growth hormone (hGH) caused increase in levels of WAP mRNA. In contrast to PRL, hGH showed its maximum effect at 0.5 μg/ml. Beyond this concentration, the effect of hGH was greatly reduced. These results suggest that hGH has a narrow optimal concentration range with regard to the stimulation of WAP expression in HC11 cells.

Corresponding author

Register with J-STAGE for free!