Journal of Reproduction and Development
Online ISSN : 1348-4400
Print ISSN : 0916-8818
ISSN-L : 0916-8818
Original Articles
Detection of Early Pregnancy Factor (EPF) in Mare Sera
Katsuhiko OHNUMAKazuei ITOYho-Ichi MIYAKEJutaro TAKAHASHIYasuhisa YASUDA
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JOURNAL FREE ACCESS

1996 Volume 42 Issue 1 Pages 23-28

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Abstract
Early Pregnancy Factor (EPF) has been detected in the pregnant sera of almost all the species examined. Yet no EPF of mare sera had been reported prior to this study, because the method of examination had not yet been established. The present study was carried out to examine various factors which influence the rosette inhibition test of mares and to establish a reliable assay system for Rosette Inhibition Titre (RIT). The following items were found optimal for the detection of RIT in mare sera: human erythrocytes (red blood cells) showed the best reactivity among human, sheep, and rat erythrocytes; the ratio of lymphocytes to erythrocytes of 1:20 was proven to show better results; and Guinea Pig Complement (GPC) was not necessary. When the rosette inhibition test was performed on 10 pregnant and 9 nonpregnant sera, the RIT values ranged from 6 to 8 (7.2 ± 1.0) in nonpregnant sera, and from 9 to 12 (9.5 ± 1.0) in pregnant sera, with a significant difference (p<0.01). These results suggested the availability of the RIT for pregnant mare sera as an index of early detection of pregnancy in mare.
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© 1996 Society for Reproduction and Development

This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license.
https://creativecommons.org/licenses/by-nc-nd/4.0/
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