Department of Reproductive and Developmental Biology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan
Kunihiko NAITO
Department of Reproductive and Developmental Biology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan
Eimei SATO
Department of Reproductive and Developmental Biology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan
The AKR/N mouse eggs fertilized in vitro with ICR mouse epididymal spermatozoa were cultured in a phosphate-free medium. At 96 h after insemination, 48% of the fertilized 1-cell embryos developed to the ≥morula stage. When a total of 96 blastocysts obtained 96-100 h after insemination was transferred to 6 ICR mice, 52 (54%) implantation sites and 27 (28%) fetuses without detectable abnormality were observed in the uterine horns on Day 17.
