A Comparative Study of the 5' Flanking Sequences of the αS1- and β-Casein Genes: Human and Other Mammalian Species

Yoshihiro FUJIWARA; Masami MIWA; Takashige SUZUKI; Masatsugu UEDA

doi:10.1262/jrd.43.333

Research Notes

A Comparative Study of the 5' Flanking Sequences of the αS1- and β-Casein Genes: Human and Other Mammalian Species

Yoshihiro FUJIWARA, Masami MIWA, Takashige SUZUKI, Masatsugu UEDA

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Keywords: Milk protein genes, Yeast artificial chromosome, Bubble-vector PCR, Sequence homology, Promoter

JOURNAL FREE ACCESS

1997 Volume 43 Issue 4 Pages 333-338

DOI https://doi.org/10.1262/jrd.43.333

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Abstract

The 5' flanking region (1.2 kb) and the first exon and intron (1.5 kb) of the human αS1-casein (CSN1) gene, and the 5' flanking sequence (2.0 kb) of the human β-casein (CSN2) gene were cloned and sequenced. Strong sequence homology was observed between the 5' flanking regions of the casein genes of humans and other mammals. Several transcriptional regulatory elements (GRE, C/EBP, YY1, STAT5) identified in the rat β-casein gene were conserved in both human casein genes.

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