Factors Affecting the Producing Efficiency of Transgenic Rats

Abstract

Transgenic rats were produced by pronuclear microinjection with 42 kinds of exogenous DNAs. The producing efficiency of transgenic rats ranged from 0.73 to 3.53% of the microinjected eggs. Promoter region, structural gene, chain length, and strand ends of the exogenous DNA was not involved in the producing efficiency of transgenic rats. The exogenous DNA can be prepared at 5 μg/ml with better efficiency than the lower and higher concentrations. Rat strain for the pronuclear eggs was a possible factor to produce transgenic rats. These results provide fundamental knowledge to produce transgenic rats effectively.