1998 Volume 44 Issue 1 Pages 101-105
To determine a site producing a low molecular weight haemagglutinin isolated from the miniature swine sperm surface, Western blot and immunohistochemistry with boar tissue extracts and paraffin sections were employed. Using anti-13K and anti-16K sera raised against 13K and 16K-proteins purified by electroelution, immunoblotting clearly showed that out of six different tissue extracts and two secretory fluids, only seminal vesicular extract and seminal vesicular fluid were positive to these antibodies. Immunohistochemistry also demonstrated that the cytoplasm of epithelial cells of seminal vesicle and secretory materials seen above the epithelial cells were strongly stained with these antisera. The present data indicated that 13K and 16K-proteins were synthesized in the seminal vesicle and then bound to sperm surface in male reproductive tracts.
