Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba Goats (Capra hircus var Shiba)

Yong-Jin Kim; Keitaro Yamanouchi; Takamasa Uekita; Toru Sawasaki; Hideaki Tojo; Chikashi Tachi

doi:10.1262/jrd.45.135

Original Articles

Molecular Cloning of cDNA for Caprine βig-h3, a TGF-β Responsive Gene, and its Expression in the Uterus of Shiba Goats (Capra hircus var Shiba)

Yong-Jin Kim, Keitaro Yamanouchi, Takamasa Uekita, Toru Sawasaki, Hideaki Tojo, Chikashi Tachi

Author information

Yong-Jin Kim
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Keitaro Yamanouchi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Takamasa Uekita
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Toru Sawasaki
The Experimental Station for Bio-Animal Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Iwama-chi, Ibaraki-ken 319-02, Japan
Hideaki Tojo
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Chikashi Tachi
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Laboratory of Applied Genetics, Department of Animal Resource Science, School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, and Laboratory of Developmental and Reproductive Biotechnology, School of Veterinary Medicine and Life Science, Azabu University, 1-17-71 Fuchinobe, Sagamihara-shi, Kanagawa-ken 229-0035, Japan

Keywords: βig-h3, Uterus, Placenta, TGF-β responsive gene, Shiba goat

JOURNAL FREE ACCESS

1999 Volume 45 Issue 2 Pages 135-141

DOI https://doi.org/10.1262/jrd.45.135

Details

Abstract

We cloned cDNA of βig-h3, a TGF-β responsive gene, from the caprine uterus by RT-PCR method combined with the 5'- and 3'- RACE. The caprine βig-h3 cDNA contains a single open reading frame (ORF) encoding a 683-amino-acid protein which is highly conserved among the three species so far examined, i. e., goats, mice and humans. The hydropathy plots of caprine p68^βig-h3 (formerly βIG-H3) protein reveals that hydrophilic and hydrophobic domains are regularly repeated. The hydrophobic domain spanning from Met¹-Ala²³ reprents the signal peptide sequence indicating that caprine p68^βig-h3, like in other species, is a secretory protein. On Northern blot analysis, the pregnant uterus exhibited a strong signal at 2.7 kb, while in the immature uterus and the placenta, signal levels were very low.

Corresponding author

Register with J-STAGE for free!