Effect of RNA Interference of BID and BAX mRNAs on Apoptosis in Granulosa Cell-derived KGN Cells

Takafumi SAI; Fuko MATSUDA; Yasufumi GOTO; Akihisa MAEDA; Miki SUGIMOTO; Hong-Mei GAO; Abul Khair Mohammad Ahan KABIR; Jun-You LI; Noboru MANABE

doi:10.1262/jrd.11-121H

Original Article

Effect of RNA Interference of BID and BAX mRNAs on Apoptosis in Granulosa Cell-derived KGN Cells

Takafumi SAI ^†, Fuko MATSUDA ^†, Yasufumi GOTO ^†, Akihisa MAEDA ^†, Miki SUGIMOTO ^†, Hong-Mei GAO ^†, Abul Khair Mohammad Ahan KABIR ^†, Jun-You LI ^†, Noboru MANABE ^†

Author information

Takafumi SAI ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Fuko MATSUDA ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Yasufumi GOTO ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Akihisa MAEDA ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Miki SUGIMOTO ^†
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
Hong-Mei GAO ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Abul Khair Mohammad Ahan KABIR ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Jun-You LI ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Noboru MANABE ^†
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
Research Center for Food Safety, The University of Tokyo, Tokyo 113-8657, Japan
Author contributions

†These authors contributed equally: Takafumi SAI, Fuko MATSUDA, Yasufumi GOTO, Akihisa MAEDA, Miki SUGIMOTO, Hong-Mei GAO, Abul Khair Mohammad Ahan KABIR, Jun-You LI, Noboru MANABE

Keywords: BCL-2-associated X protein (BAX), BH3-interacting domain death agonist (BID), Mitochondrion-dependent type II apoptosis, RNA interference (RNAi)

JOURNAL FREE ACCESS

2012 Volume 58 Issue 1 Pages 112-116

DOI https://doi.org/10.1262/jrd.11-121H

View "Advance Publication" version (November 4, 2011).

Details

Abstract

In mitochondrion-dependent type II apoptosis, BH3-interacting domain death agonist (BID) and BCL-2-associated X protein (BAX) promote death ligand and receptor-mediated cell death. In porcine ovaries, the levels of BID and BAX increase in follicular granulosa cells during atresia. In the present study, to confirm the pro-apoptotic activity of BID and BAX in granulosa cells, we examined the effect of RNA interference of BID or BAX on apoptosis using a human ovarian granulosa tumor cell line, KGN. By reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, expression of BID and BAX was detected in KGN cells. Then, we suppressed BID and BAX mRNA expression in KGN cells using small interfering RNA (siRNA). When BID or BAX was suppressed, a significant decrease in the apoptotic cell rate was noted. In granulosa-derived cells, BID and BAX showed pro-apoptotic activity. These results suggest that BID and BAX act as signal-transducing factors in mitochondrion-dependent type II apoptosis.

Corresponding author

Register with J-STAGE for free!