IRS2 depletion inhibits cell proliferation and decreases hormone secretion in mouse granulosa cells

Lanjie LEI; Feng HAN; Qiuyan CUI; Weifang LIAO; Hui LIU; Gaopeng GUAN; Lei YANG

doi:10.1262/jrd.2018-055

Original Article

IRS2 depletion inhibits cell proliferation and decreases hormone secretion in mouse granulosa cells

Lanjie LEI, Feng HAN, Qiuyan CUI, Weifang LIAO, Hui LIU, Gaopeng GUAN, Lei YANG

Author information

Lanjie LEI
Clinical Skills Center, Affiliated Hospital of Jiujiang University, Jiujiang University, Jiangxi 332000, China
Feng HAN
Clinical Skills Center, Affiliated Hospital of Jiujiang University, Jiujiang University, Jiangxi 332000, China
Qiuyan CUI
Clinical Skills Center, Affiliated Hospital of Jiujiang University, Jiujiang University, Jiangxi 332000, China
Weifang LIAO
Clinical Skills Center, Affiliated Hospital of Jiujiang University, Jiujiang University, Jiangxi 332000, China
Hui LIU
Clinical Skills Center, Affiliated Hospital of Jiujiang University, Jiujiang University, Jiangxi 332000, China
Gaopeng GUAN
Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiangxi 332000, China
Lei YANG
College of Basic Medical, Jiujiang University, Jiangxi 332000, China
Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiangxi 332000, China

Keywords: AKT, Cell proliferation, Granulosa cells, Insulin receptor substrate 2 (IRS2), Steroidogenesis

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Supplementary material

2018 Volume 64 Issue 5 Pages 409-416

DOI https://doi.org/10.1262/jrd.2018-055

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Abstract

Insulin receptor substrate 2 (IRS2) is a component of the insulin/insulin-like growth factor 1 (IGF1) signaling cascade, which plays an important role in mouse hypothalamic and ovarian functions. The present study was conducted to investigate the role of IRS2 in steroidogenesis, apoptosis, cell cycle and proliferation in mouse granulosa cells (GCs). Flow cytometry and CCK8 assay showed that IRS2 knockdown inhibited cell proliferation, reduced cell viability, and increased apoptosis in GCs. The study also revealed that the expression of Cyclin A1, Cyclin B1 and Bcl2 was downregulated, while the expression of Bax, Cyclin D1 and Cyclin D2 was upregulated. ELISA analysis showed that IRS2 knockdown decreased the concentrations of estradiol (E₂) and progesterone (P₄), which was further validated by the decreased expression of Star, Cyp11a1, and Cyp19a1. Moreover, IRS2 knockdown altered the expression of Has2 and Ptgs2, which are essential for folliculogenesis. In addition, we found that IRS2-mediated cell viability and hormone secretion are dependent on the PI3K/AKT signaling pathway. Collectively, this study demonstrated that IRS2 plays an important role in the regulation of cell proliferation and steroidogenesis in mouse GCs via the PI3K/AKT signaling pathway.

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