Rescue In Vitro Fertilization Method for Legacy Stock of Frozen Mouse Sperm

Naomi NAKAGATA; Toru TAKEO; Kiyoko FUKUMOTO; Yukie HARUGUCHI; Tomoko KONDO; Yumi TAKESHITA; Yuko NAKAMUTA; Tomoko UMENO; Shuuji TSUCHIYAMA

doi:10.1262/jrd.2013-141

This article has now been updated. Please use the final version.

Rescue In Vitro Fertilization Method for Legacy Stock of Frozen Mouse Sperm

Naomi NAKAGATA, Toru TAKEO, Kiyoko FUKUMOTO, Yukie HARUGUCHI, Tomoko KONDO, Yumi TAKESHITA, Yuko NAKAMUTA, Tomoko UMENO, Shuuji TSUCHIYAMA

Author information

Naomi NAKAGATA
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Toru TAKEO
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Kiyoko FUKUMOTO
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Yukie HARUGUCHI
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Tomoko KONDO
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Yumi TAKESHITA
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Yuko NAKAMUTA
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Tomoko UMENO
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan
Shuuji TSUCHIYAMA
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan

Keywords: C57BL/6, Cryopreserved sperm, In vitro fertilization, Mouse, Transport

JOURNAL FREE ACCESS Advance online publication

Article ID: 2013-141

DOI https://doi.org/10.1262/jrd.2013-141

The final version of this article is now available: Vol. 60 (2014), No. 2 pp. 168-171

Details

Abstract

Sperm cryopreservation has been widely adopted for maintenance of the genetically engineered mouse (GEM). The cryopreserved sperm are being exchanged among many institutes worldwide. However, the recipients are not always able to obtain high fertilization rates with the frozen sperm shipped from senders. In this study, we cryopreserved mouse sperm via various methods and performed in vitro fertilization (IVF) in which the combination of methyl-beta-cyclodextrin for sperm preincubation and reduced glutathione for insemination was used (the MBCD-GSH IVF). In addition, frozen sperm sent from the Jackson Laboratory (USA) were thawed and used for IVF in the same manner. The fertilization rates of both the sperm cryopreserved via the methods applied in some countries and the cryopreserved GEM sperm improved when used with the MBCD-GSH IVF method. Therefore, we strongly believe that the MBCD-GSH IVF method brings about relatively high fertilization rates with any strain of frozen mouse sperm.

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