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Journal of Reproduction and Development
Article ID: 2013-141

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http://doi.org/10.1262/jrd.2013-141

  Advance Publication  

Rescue In Vitro Fertilization Method for Legacy Stock of Frozen Mouse Sperm

Naomi NAKAGATA1), Toru TAKEO1), Kiyoko FUKUMOTO1), Yukie HARUGUCHI1), Tomoko KONDO1), Yumi TAKESHITA1), Yuko NAKAMUTA1), Tomoko UMENO1), Shuuji TSUCHIYAMA1)

1) Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan

[Advance Publication] Released 2014/02/04
received 2013/12/15   accepted 2014/01/03

Keywords: C57BL/6, Cryopreserved sperm, In vitro fertilization, Mouse, Transport

The final version of this article with its full bibliographical information is available. To access the article, click here (Vol. 60 (2014), No. 2 pp. 168-171).


Sperm cryopreservation has been widely adopted for maintenance of the genetically engineered mouse (GEM). The cryopreserved sperm are being exchanged among many institutes worldwide. However, the recipients are not always able to obtain high fertilization rates with the frozen sperm shipped from senders. In this study, we cryopreserved mouse sperm via various methods and performed in vitro fertilization (IVF) in which the combination of methyl-beta-cyclodextrin for sperm preincubation and reduced glutathione for insemination was used (the MBCD-GSH IVF). In addition, frozen sperm sent from the Jackson Laboratory (USA) were thawed and used for IVF in the same manner. The fertilization rates of both the sperm cryopreserved via the methods applied in some countries and the cryopreserved GEM sperm improved when used with the MBCD-GSH IVF method. Therefore, we strongly believe that the MBCD-GSH IVF method brings about relatively high fertilization rates with any strain of frozen mouse sperm.

Copyright © 2014 Society for Reproduction and Development
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