Abstract
Effects of sugar, glycine, milk and egg-yolk in the calcium-free Krebs Ringer-phosphate on the liability of dog spermatozoa stored at 4°C were studied.
1. It was indicated that the calcium-free KRP buffer was less available on the storage of dog spermatozoa and the longest survival period of dog spermatozoa were seven days in treatments.
2. The sugers were less effective on the viability of diluted dog spermatozoa in the calcium-free KRP buffer.
3. Egg yolk-calcium-free KPR buffer was superior to the milk-calcium-free KRP buffer improving for the storage of dog spermatozoa.
4. Glycine was seemed to harmful on the survival of dog spermatozoa without egg-yolk extenders.
