Abstract
The composition of semen extenders was studied for the strage of dog spermatozoa at 4°C.
The results obtained are as follows:
1. The basic buffer containing magnesium chloride, potassium chloride, sodiummonohydrogen phosphate and sodium chloride was used. Extenders were composed by adding 20 percent egg-yolk or 50 percent milk to the basic buffer.
It was found after the systematic studies with dog semen extended 1 : 9 and stored at 4°C that. the best motility index after dilution was 74, and that survival period was three days in Experiment I. Survival of dog spermatozoa in the sodium citrate extender was not so longer, though survival period was prolonged by four days.
2. The basic extender in Experiment II was contained with potassium chloride, sodiummonohydrogen phosphate and sodium citrate. Dog spermatozoa could survive for the longest period in the extender added by 1g of peptone, 0.75g of glycine and 10% of eggyolk to the 100 ml of the basic buffer. The longest survival period was 16 days in Experiment II, but motility was decrined to a half after one day.
3. The effect of addition of chlorpromazine for the storage of dog spermatozoa was examined in Experiment III with the extender of Experiment II. The survival of dog spermatozoa stored in extender containing chlorpromazine in concentration of 5 mg/100 ml was prolonged than in the extender containing no chlorpromazine.
