The effect of synthetic LH-Releasing Hormone (LH-RH) on ovulation in adult and immature rats
1974 Volume 20 Issue 3 Pages 101-104
Details
1974 Volume 20 Issue 3 Pages 101-104
Effects of 2 synthetic LH-RH (decapeptide amide-p-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2: (Gn-RH), nonapeptide-des-Gly-NH210, Proethylamide9: (Gn-RH-I) on ovulation of adult and immature rats were investigated.
Results obtained were as follows: 1) In adults, LH-RH disolved in 0.5 mlsaline was injected i. v. at 2:00 PM of diestrus or metestrus day.
Rats were killed on the following day after LH-RH injection on diestrus day and on 4 days after the injection of metestrus day to examine the ampulla for ova.
i) Ovulation was observed in rats injected with more than 2 μg of Gn-RH or more than 0.2 μg of Gn-RH-I on diestrus day.
ii) When 50 μg of Gn-RH or more than 2 μg of Gn-RH-I was injected, ovulation was delayed 1 day.
2) In immature rats aged 2427 days, 3 IU of PMS was injected s. c. at 9:3010:00 AM to induce ovulation. Then clomiphene (Clomiphene-citrate: 5 mg per 100 g B. W. in 30% ethanol, after 6 hrs of PMS treatment) or Nembutal (4 mg per 100 g B. W., after 49 hrs of PMS treat-ment) was injected to block ovulation.
i) In clomiphene treated rats, ovulation was induced by more than 0.2 μg of Gn-RH or Gn-RH-I.
ii) In Nembutal treated rats, ovulation was induced by more than 2 pg of Gn-RH or more than 0.2 μg of Gn-RH-I.
