Isolation of rat immunoglobulins and determination of immunogiobulins in rat uterine fluid.
1977 Volume 22 Issue 4 Pages 139-144
Details
1977 Volume 22 Issue 4 Pages 139-144
Present study was designed to isolate immunoglobulins from serum and colostrum whey, and determine immunoglobulins in uterine fluid of proestrous rat.
Results obtained were as follows:
1. Subclass of IgG and IgA in serum and colostrum whey were obtained by DEAE cellulose, sephadex-G 200 and sepharose-6B column chromatography (Figs. 13).
2. Physicochemical analysis of immunoglobulins were performed by SDS acrylamide gel electro-phoresis and density gradient ultracentrifugation methods (Figs. 4, 5). Molecular weight of isolated IgG and IgA was about 16 × 104 and 50 × 104, respectively.
3. Immunoglobulins in uterine fluid were determined by immunoelectrophoresis. Although high molecular IgA in uterine fluid determined by physicochemical analysis was very small amount, uterine fluid contained high level of the protein component reacted to serum IgA, of which molecular weight is smaller than 20 × 104.
Immunoglobulin G was not appeared in the rat uterine fluid at 22:00 of proestrus.
