Successfull pregnancy using boar spermatozoa stored at 4°C for 12 days

Abstract

The sperm-rich fraction of semen was collected from two Landrace boars by the manual method. After removal of seminal plasma by centrifugation at room temperature spermatozoa were resuspended in ETCG-catalase diluent (Table 1) to give a concentration of 1-2 × 10⁸ cells per milliliter, then cooled and stored in a cold room (4°C) for 1014 days. The sperm suspension (50100 ml) was inseminated in the normal way on the 2nd day of estrus. Of the two gilts inseminated, one farrowed 11 normal piglets following single insemination with sperm suspension stored for 12 days and the other gilt returned to estrus 19 days after insemination. Of the three sows, two returned to estrus 4966 days and one sow 22 days after insemination.