Abstract
A new microflushing analysis method for sperm motility is proposed. Conventionally, spermatozoa have been immersed in mineral oil and manipulated without exposure to the air. In this method, since the spermatozoa scarcely adhere to the surface of the slide glass during microscopic observation of sperm motility, usually over 90% of the motility is maintained. Furthermore, it became possible to detect and characterize the flagellar bend newly formed in a different test reagent. Because this method requires only an extremely small amount of spermatozoa for assay, repeated assay even for small animals is possible. Therefore, the microflushing method is considered to be one of the best method for assaying sperm motility.
