Abstract
The reliability of DNA fingerprinting in evaluating the parent/offspring relationship (pedigree) of 5 related hybrid dogs (parents: 2; offspring: 3) and 2 non-related Beagle breeds was investigated with the Myo probe. DNA was isolated from leucocyte, digested with Hinf I before subjecting to agarose gel electrophoresis and transferred to a nylon membrane eventually. The nylon membrane was hybridized with 32P-labeled Myo probe, and the respective bands were detected after autoradiography. Bands derived from the 3 offspring resembled that of either the father or mother. This clearly indicates that DNA fingerprinting can be effectively employed for pedigree evaluation, and may serve as a positive proof of paternity as well.
