Study of EPF-Like Substance(s) Detected in Fertilized Bovine Ovum Culture Medium

Yoshimi IKEMIZU; Kazuei ITO; Kyoko KAWAHATA; Taichi GOTO; Jutaro TAKAHASHI; Yasuhisa YASUDA

doi:10.1262/jrd.40.7

Yoshimi IKEMIZU, Kazuei ITO, Kyoko KAWAHATA, Taichi GOTO, Jutaro TAKAHASHI, Yasuhisa YASUDA

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Keywords: Early pregnancy factor (EPF), Ovum factor (OF), Fertilized ova, Bovine, Rosette inhibi-tion titer (RIT)

JOURNAL FREE ACCESS

1994 Volume 40 Issue 1 Pages 7-11

DOI https://doi.org/10.1262/jrd.40.7

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Abstract

This study was undertaken to confirm whether early pregnancy factor (EPF)-like substance(s) was secreted by fertilized bovine ova. Using the rosette inhibition test, EPF-like substance(s) was detected in the culture media of fertilized bovine ova, while this substance was not found in the culture media of unfertilized bovine ova. EPF-like activity, analyzed by HPLC-gel permeation chromatography, was present between molecular weights of 16 and 37 KD. In conclu-sion, EPF-like substance(s) is secreted by fertilized bovine ovum and has a molecular weight of16-37 KD.

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