Journal of Reproduction and Development
Online ISSN : 1348-4400
Print ISSN : 0916-8818
ISSN-L : 0916-8818
Nucleotide Sequence of eCG α-Subunit cDNA and Its Expression in the Equine Placenta
Kwan-Sik MINMikihiko SHINOZAKIKiyoshi MIYAZAWARyohei NISHIMURANobuo SASAKIKunio SHIOTATomoya OGAWA
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1994 Volume 40 Issue 4 Pages 301-305

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Abstract

Equine chorionic gonadotropin (eCG) is a member of the glycoprotein family, which includes lutropin (LH), follitropin (FSH), and thyrotropin (TSH), all consisting of two noncovalently linked a-and β-subunits. In the present study, we determined the full length of the equine α-subunit cDNA sequence. mRNA was extracted from equine placenta on day 70 of gestation. The nucleotide sequence of the eCG a-subunit cDNA was amplified by PCR using mixed primers designed on the basis of the nucleotide sequence of human, rat and mouse a-subunits. Analysis of the nucleotide sequence revealed that the a-subunit amplified contained 387 bp which included the signal peptide (24 amino acids containing 4 amino acids expected) and the first 3 amino acid residues. When the expression of both subunit mRNAs in the equine placenta was compared by Northern blotting, the expression of the β-subunit mRNA was relatively greater than that of the a-subunit.

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© Japanese Society of Animal Reproduction
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