Inhibition of γ-Radiation Induced DNA Damage in Plasmid pBR322 by TMG, a Water-soluble Derivative of Vitamin E

REMA RAJAGOPALAN; KHALIDA WANI; NAGARAJ G. HUILGOL; TSUTOMU V. KAGIYA; CHERUPALLY K. KRISHNAN NAIR

doi:10.1269/jrr.43.153

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Inhibition of γ-Radiation Induced DNA Damage in Plasmid pBR322 by TMG, a Water-soluble Derivative of Vitamin E

REMA RAJAGOPALAN, KHALIDA WANI, NAGARAJ G. HUILGOL, TSUTOMU V. KAGIYA, CHERUPALLY K. KRISHNAN NAIR

著者情報

キーワード: DNA strandbreaks, Thymine glycol, Radiation protection, Tocopherol Monoglucoside

ジャーナルフリー

2002 年 43 巻 2 号 p. 153-159

DOI https://doi.org/10.1269/jrr.43.153

詳細

抄録

Alpha-tocopherol monoglucoside (TMG), a water-soluble derivative of α-tocopherol, has been examined for its ability to protect DNA against radiation-induced strand breaks. Gamma radiation, up to a dose of 6 Gy (dose rate, 0.7 Gy/ minute), induced a dose-dependent increase in single strand breaks (SSBs) in plasmid pBR322 DNA. TMG inhibited the formation of γ-radiation induced DNA single strand breaks (SSBs) in a concentration-dependent manner; 500 μM of TMG protected the single strand breaks completely. It also protected thymine glycol formation induced by γ-radiation in a dose-dependent manner, based on an estimation of thymine glycol by HPLC.

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