Effects of Cell Concentrations on the Survival and Repopulation of Haemopoietic Stem Cells in Irradiated Bone Marrow Cell Culture in vitro

抄録

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells after irradiation were studied in the long-term culture of mouse bone marrow cells in vitro.
No difference was observed in the survival of the stem cells among cultures in which 0-10⁷ cells were re-inoculated on the adherent cell colonies in the culture flask. Stem cells showed a significant proliferation within 1 week and the number of the stem cells exceeded the control in 3 weeks after irradiation in the cultures with less than 10⁶ re-inoculated cells per flask. In contrast, there was a considerable delay in the onset of stem cell proliferation after irradiation in the culture with 107 cells per flask.
Based on these results, a possiblity that a stimulator of stem cell proliferation, released from irradiated stromal cells, is cancelled by an inhibitory factor produced by irradiated or unirradiated haemopoietic cells is postulated.