⁵⁹Fe Release from Alveolar Macrophages Ingested ⁵⁹Fe-iron Dextran —Enhancement by Combination of Ca-DTPA and Macrophage Activating Substances—

Abstract

Effect of calcium diethylenetriaminepentaacetate (Ca-DTPA) and macrophage activating substances, lipopolysaccharide (LPS) and zymosan, on ⁵⁹Fe release from rabbit alveolar macrophages which ingested ⁵⁹Fe-iron dextran was studied in vitro. The alveolar macrophages harvested by lung lavage, loaded with the ⁵⁹Fe-iron dextran were cultured in Hanks'' balanced salt solution (HBSS) at 37°C for 8 hrs with or without the agents. With the addition of 10 mM Ca-DTPA, the ⁵⁹Fe release was increased to about 4-fold of the control level. Effect of LPS or zymosan was not as large as that of Ca-DTPA, but the simultaneous use of LPS or zymosan with Ca-DTPA enhanced the more elevated ⁵⁹Fe release.