Abstract
The cellular uptake of Adriamycin (ADR) by solid tumors consisting of Ehrlich ascites tumor cells (EATC) was investigated. The intracellular levels of ADR in the EATC were measured by flow cytometry. The EATC tumors were heated at various temperatures for 30 min after the in vivo administration of ADR to the mice into which the EATC were inoculared. The amount of intracellular ADR in the EATC excised from the mice treated with 40°C hyperthermia was increased by 80% over that determined for those treated at body temperature. However, the quantity of ADR in the EATC excised from mice which received 43°C hyperthermia was similar to that for those treated at body temperature. These results indicate that combined therapy using hyperthermia and ADR has both synergic and additive effects, depending on the temperatures used for hyperthermia.
