抄録
We established a technique based on single cell gel electrophoresis (comet assay) to detect DNA damage in mouse spleen cells induced by low dose of ionizing radiation. C57BL/6N female mice, 6-week old, were irradiated with 0.5Gy of X-rays at 1.6Gy/min or 137Cs gamma-rays at 1.2mGy/hr. After the irradiation, spleen of the mouse was removed and cell suspension prepared. The amount of DNA damage was measured by the comet assay. The amount of DNA damage of the low dose rate irradiation was smaller than the high dose rate irradiation ; however, it was slightly larger than that in control mice. [J Radiat Res 44:393 (2003)]
