主催: 日本放射線影響学会
We previously demonstrated that scid mouse cells were hypersensitive to genetic instability induced by radiation. To confirm this result, we established scid mouse cells expressing human DNA-PKcs by the introduction of a human chromosome 8. The human DNA-PKcs complemented the hypersensitivity to the induction of delayed reproductive death in scid mouse cells, indicating that NHEJ play a role to suppress the induction of genetic instability. To know a role of double strand break repair in the induction of genetic instability further, we investigated delayed reproductive death in chicken Rad54−/- DT40 cells. The result indicated no difference in delayed reproductive death between wild-type and Rad54−/- DT40 cells, suggesting that homologous recombination might play little role in the induction of delayed reproductive death. [J Radiat Res 44:441 (2003)]