抄録
Radiation generates reactive oxygen species (ROS) that may contribute to the induction of genetic instability. We examined the suppressive effect of a radical scavenger, ascorbic acid phosphate magnesium salt (APM), on the induction of delayed reproductive cell death by radiation. The delayed cell death was determined by two successive colony formation periods. The treatment with APM was applied either primary and secondary colony formation. The result indicated that the APM treatment during primary colony formation, but not secondary colony formation, suppressed the delayed reproductive cell death. We also demonstrated that the rapid increase and then decrease of the amount of hydrogen peroxide (ROS) in X-ray-irradiated cells by 5 hr postirradiation and then the level of hydrogen peroxide gradually decrease to a base line within two weeks. The APM treatment kept the hydrogen peroxide production in a lower level than an untreated control. These results suggest that the cause of genetic instability might be fixed by partly ROS during 2 weeks postirradiation. [J Radiat Res 44:442 (2003)]
