抄録
BALB/c mice are susceptible to radiation-induced lymphomagenesis, while STS mice are resistant. Previously we showed that more than one locus could be associated with the strain difference. By the use of a series of congenic lines with different portions of STS-derived chromosome 4 in the BALB/c background we also showed that lymphoma susceptibility was linked to the proximal half of chromosome 4. In the present study, we performed linkage analysis for susceptibility to lymphomagenesis using (BALB/c x STS)F1 x BALB/c mice. Only females were used and subjected to 4 x 1.7 Gy of X-irradiation. Development of lymphomas was observed during 300 days after irradiation. Genotyping was carried out with PCR and PAGE using microsatellite markers. Linkage was analyzed by the Kaplan-Meier method. Significant linkage was detected both at D4Mit17 and in the D4Mit302-D4Mit255 segment more than 10 cM distal to D4Mit17 on chromosome 4. Analysis of congenic lines identified a susceptibility locus in the D4Mit302-D4Mit255 segment, but not in the D4Mit86-D4Mit302 segment between the two putative susceptibility loci. Further analysis of congenic lines is presently under way.
